diff --git a/biology/Makefile b/biology/Makefile
index 192c77a0d63f..3f12e044804a 100644
--- a/biology/Makefile
+++ b/biology/Makefile
@@ -108,6 +108,7 @@
     SUBDIR += mafft
     SUBDIR += mapm3
     SUBDIR += mashmap
+    SUBDIR += mca-calling
     SUBDIR += megahit
     SUBDIR += metaeuk
     SUBDIR += migrate
diff --git a/biology/mca-calling/Makefile b/biology/mca-calling/Makefile
new file mode 100644
index 000000000000..5f2afd08795f
--- /dev/null
+++ b/biology/mca-calling/Makefile
@@ -0,0 +1,27 @@
+PORTNAME=	mca-calling
+DISTVERSION=	0.1.0
+CATEGORIES=	biology
+MASTER_SITES=	# empty
+
+MAINTAINER=	jwb@FreeBSD.org
+COMMENT=	Core tools for Mosaic Chromosomal Alteration event calling
+WWW=		https://github.com/auerlab/
+
+LICENSE=	BSD2CLAUSE
+
+RUN_DEPENDS+=	sra-tools>0:biology/sra-tools
+RUN_DEPENDS+=	bcftools>0:biology/bcftools
+RUN_DEPENDS+=	samtools>0:biology/samtools
+RUN_DEPENDS+=	vcf-split>0:biology/vcf-split
+RUN_DEPENDS+=	ad2vcf>0:biology/ad2vcf
+RUN_DEPENDS+=	vcf2hap>0:biology/vcf2hap
+RUN_DEPENDS+=	haplohseq>0:biology/haplohseq
+RUN_DEPENDS+=	bio-mocha>0:biology/bio-mocha
+RUN_DEPENDS+=	bedtools>0:biology/bedtools
+RUN_DEPENDS+=	mawk>0:lang/mawk
+RUN_DEPENDS+=	curl>0:ftp/curl
+RUN_DEPENDS+=	liblz4>0:archivers/liblz4
+
+USES=		metaport
+
+.include <bsd.port.mk>
diff --git a/biology/mca-calling/distinfo b/biology/mca-calling/distinfo
new file mode 100644
index 000000000000..d649a5847c66
--- /dev/null
+++ b/biology/mca-calling/distinfo
@@ -0,0 +1 @@
+TIMESTAMP = 1729776668
diff --git a/biology/mca-calling/pkg-descr b/biology/mca-calling/pkg-descr
new file mode 100644
index 000000000000..23486297ac55
--- /dev/null
+++ b/biology/mca-calling/pkg-descr
@@ -0,0 +1,5 @@
+The mca-calling meta-port provides the core tools needed for performing
+a typical mCA (Mosaic Chromosomal Alteration) analysis, including
+conversion of SRA VCF files to a format suitable for haplohseq and
+MoCha, and haplohseq and MoCha event calling.  The GWAS analysis that
+follows is typically done with R-based tools installed within R.