diff --git a/biology/bedtools/Makefile b/biology/bedtools/Makefile
index 7e8b367384c3..4f0b519d87f5 100644
--- a/biology/bedtools/Makefile
+++ b/biology/bedtools/Makefile
@@ -1,6 +1,6 @@
 PORTNAME=		bedtools
 DISTVERSIONPREFIX=	v
-DISTVERSION=		2.31.0
+DISTVERSION=		2.31.1
 CATEGORIES=		biology
 
 MAINTAINER=	jwb@FreeBSD.org
diff --git a/biology/bedtools/distinfo b/biology/bedtools/distinfo
index ad0fff26a477..eb599d15d598 100644
--- a/biology/bedtools/distinfo
+++ b/biology/bedtools/distinfo
@@ -1,3 +1,3 @@
-TIMESTAMP = 1682717508
-SHA256 (arq5x-bedtools2-v2.31.0_GH0.tar.gz) = 183cf9a96aabc50ef4bd557a53fd01557a123c05a0dc87651371878f357439ec
-SIZE (arq5x-bedtools2-v2.31.0_GH0.tar.gz) = 21085982
+TIMESTAMP = 1699454464
+SHA256 (arq5x-bedtools2-v2.31.1_GH0.tar.gz) = 79a1ba318d309f4e74bfa74258b73ef578dccb1045e270998d7fe9da9f43a50e
+SIZE (arq5x-bedtools2-v2.31.1_GH0.tar.gz) = 21085970